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Standard operating procedure for pH meter

 1). pH meters -  Objective:                      To demonstrate how to use a standard pH meter in the TRACES Centre and undergraduate laboratories. This process explains how to accurately test a solution's pH.  2. Scope: Digital pH meters in the TRACES Centre and the UG Laboratory are affected. These devices include (but are not limited to) Sartorius, Oakton, and Mettler-Toldeo.  3. Responsibility :   Designated persons in the research lab are responsible for training employees and students on this protocol and ensuring that it is followed. It is the responsibility of selected persons (Staff or Students) to adhere to the procedure's guidelines.  4. Equipment :  • pH meter, digital  • Double-junction Ag/AgCl electrode pH electrode  • ATC probe (automatic temperature control) (if available)  • Transfer utensils, pipettes, spatula or capillaries  • Clean stir bars on a stir p...
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CHEMICAL TEST FOR ANTHRAQUINONE GLYCOSIDES

 CHEMICAL TEST FOR ANTHRAQUINONE GLYCOSIDES 


 1) Brontrager’s test- This test is performed for the O-glycosides. Drug is dissolved in 1ml H2SO4 and mixture is boiled. Filter the solution, filterate is then mixed with chloroform. Chloroform layer mixed with ammonia gives rose pink color if O-glycosides are present. 


 2) Modified brontrager’s test- This test is performed for the investigation of C-glycosides. Drug is mixed with H2SO4 and FeCl3. The next procedure is same as for the O-glycosides in brontrager’s test.  


Hydroxy anthraquinones- Drug is mixed with Potassium Hydroxide. If hydroxy anthraquinones are present red color is present.

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